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counterstaining with dapi  (Beyotime)


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    Structured Review

    Beyotime counterstaining with dapi
    Counterstaining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 32249 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+counterstaining/DAPI/pm41981465-107-32-35
    Average 99 stars, based on 32249 article reviews
    counterstaining with dapi - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Incubation:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Article Title: Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke
    Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies against ZO-1 (Invitrogen, 61-7300, 1:50), NeuN (Abcam, ab104224, 1:400), or Nrf2 (Proteintech, 16396-1-AP, 1:500). .. After washing, samples were incubated with fluorophore-conjugated secondary antibodies (Invitrogen, 1:500) for 2 h at room temperature, followed by DAPI counterstaining (Beyotime, China). .. Imaging was performed using an Olympus FV3000 confocal laser scanning microscope.

    Article Title: Macrophage-mimetic liposomes co-delivering ceria and Ac2-26 peptide for penumbra protection in ischemic stroke.
    Article Snippet: Samples were incubated overnight at 4 °C with primary antibodies against ZO-1 (Invitrogen, 61-7300, 1:50), NeuN (Abcam, ab104224, 1:400), or Nrf2 (Proteintech, 16396-1-AP, 1:500). .. After washing, samples were incubated with fluorophore-conjugated secondary antibodies (Invitrogen, 1:500) for 2 h at room temperature, followed by DAPI counterstaining (Beyotime, China). .. Imaging was performed using an Olympus FV3000 confocal laser scanning microscope.

    Labeling:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    TUNEL Assay:

    Article Title: Heliox preconditioning exerts pulmonary protection effects on neonatal acute respiratory distress syndrome by inhibiting oxidative stress and apoptosis
    Article Snippet: .. After blocking, sections were incubated with transferase dUTP-mediated nick-end labeling (TUNEL) reaction mixture (50 μL/section) overnight at 37 °C, followed by DAPI counterstaining (C1005, Beyotime). ..

    Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation
    Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by DAPI counterstaining (Beyotime, #C1002). ..

    Article Title: TNIP3 Attenuates Colitis by Inhibiting the TAB1/NF-κB/NLRP3 Signaling Pathway in Macrophages.
    Article Snippet: Background Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, is characterized by chronic, recurrent, and non-specific intestinal inflammation.. This study aimed to elucidate the mechanism of TNIP3 (TNFAIP3interacting protein 3) in modulating macrophage (Mφ) polarization and intestinal epithelial barrier function in IBD.. Methods LPS-induced RAW 264.7 cells and DSS-induced mouse models were used to investigate the role of TNIP3 in IBD.

    Staining:

    Article Title: Targeted neural stem cell-derived extracellular vesicles loaded with Sinomenine alleviate diabetic peripheral neuropathy via WNT5a/TRPV1 pathway modulation
    Article Snippet: Cells were fixed with 4% paraformaldehyde (Yeasen, #60536ES60) for 15 min, permeabilized with 0.25% Triton X-100 for 20 min, and blocked with 5% BSA (Yeasen, #36101ES25). .. Staining was carried out using a TUNEL kit (Beyotime, #C1086), followed by DAPI counterstaining (Beyotime, #C1002). ..

    Fluorescence:

    Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway
    Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After DAPI counterstaining for 5 minutes and mounting with P0126 (Beyotime), apoptotic cells were quantified in five randomly selected fields under a fluorescence microscope. ..

    Microscopy:

    Article Title: Ginkgetin Alleviates Doxorubicin-Induced Heart Failure by Regulating Mitochondrial Dysfunction Through the AMPK/Sirt1/NF-κB Signaling Pathway
    Article Snippet: Samples were permeabilized with 0.1% Triton X-100 for 10 minutes prior to terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining ( C10617 , Invitrogen) at 37 °C for 1 hour. .. After DAPI counterstaining for 5 minutes and mounting with P0126 (Beyotime), apoptotic cells were quantified in five randomly selected fields under a fluorescence microscope. ..



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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Beyotime counterstaining with dapi
    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Vector Laboratories counterstain
    (a) Actin (green) and <t>DAPI</t> (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.
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    Image Search Results


    (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Journal: Bioengineering & Translational Medicine

    Article Title: Extracellular matrix microarchitecture modulates cellular behavior and extracellular vesicle phenotypes in biomimetic tendon models

    doi: 10.1002/btm2.70134

    Figure Lengend Snippet: (a) Actin (green) and DAPI (blue) stain of tendon derived cells seeded on tissue culture plate (TCP) monolayer controls, healthy mimetic models and diseased mimetic models ( n = 3). Scale bar: 100 μm. (b) Nuclear aspect ratio (NAR) was quantified using ImageJ by converting microscopic image to grayscale and measuring major and minor axes of individual nuclei. A higher major‐to‐minor ratio indicates increased cellular elongations. (c) Cells on the healthy model showed greater elongation than both the monolayer control and diseased model by day 3, while the diseased model also exceeded the monolayer control. By day 5, elongation remained elevated only in the healthy model relative to the diseased, and by day 7, the healthy group exhibited significantly greater elongation than all other models. No significant differences were observed by day 10. Significance was defined as p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), ns = not significant.

    Article Snippet: Cellular morphology was assessed via actin staining (Invitrogen, Cat. No. A12379) and nuclear counterstaining with DAPI (Fisher Scientific, Cat. No. H‐1200‐10) ( n = 3).

    Techniques: Staining, Derivative Assay, Control